human 1 cdna microarray platform Search Results


atlas human 1 2 arrays  (TaKaRa)
94
TaKaRa atlas human 1 2 arrays
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Atlas Human 1 2 Arrays, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human 1.0st gene chip assay  (Thermo Fisher)
90
Thermo Fisher human 1.0st gene chip assay
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Human 1.0st Gene Chip Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human 1 cdna microarrays  (Agilent technologies)
90
Agilent technologies human 1 cdna microarrays
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Human 1 Cdna Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human 1 cdna microarray  (Agilent technologies)
90
Agilent technologies human 1 cdna microarray
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Human 1 Cdna Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total human 1.2 cdna microarray  (Becton Dickinson)
90
Becton Dickinson total human 1.2 cdna microarray
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Total Human 1.2 Cdna Microarray, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cdna arrays (human 1  (Agilent technologies)
90
Agilent technologies human cdna arrays (human 1
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Human Cdna Arrays (Human 1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atlas glass human 1 0 microarray  (TaKaRa)
86
TaKaRa atlas glass human 1 0 microarray
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Atlas Glass Human 1 0 Microarray, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human 1 cdna microarray slides  (Agilent technologies)
90
Agilent technologies human 1 cdna microarray slides
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Human 1 Cdna Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human 1.0 st exon microarray  (Thermo Fisher)
90
Thermo Fisher human 1.0 st exon microarray
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Human 1.0 St Exon Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human 1.0 st exon array  (Thermo Fisher)
90
Thermo Fisher human 1.0 st exon array
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Human 1.0 St Exon Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atlas human 1 2 cdna arrays  (TaKaRa)
86
TaKaRa atlas human 1 2 cdna arrays
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Atlas Human 1 2 Cdna Arrays, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sureprint g3 human 1×1m microarray  (Agilent technologies)
90
Agilent technologies sureprint g3 human 1×1m microarray
Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.
Sureprint G3 Human 1×1m Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.

Journal:

Article Title: A Phosphorylated Cytoplasmic Autoantigen, GW182, Associates with a Unique Population of Human mRNAs within Novel Cytoplasmic Speckles

doi: 10.1091/mbc.01-11-0544

Figure Lengend Snippet: Identification of mRNAs associated with GW182-mRNP complexes using cDNA microarray analysis. As described in MATERIALS AND METHODS, RNA was extracted from immunoprecipitated GW182-mRNPs or total cell lysate and used to make reverse-transcribed radiolabeled probes for Atlas Human 1.2 Arrays containing 1188 singly spotted cDNA segments (CLONTECH). Phosphorimages were scanned and stored as gel files and then analyzed using ATLASIMAGE 1.01 software (CLONTECH). A default external background setting was used in conjunction with a background-based signal threshold to determine gene signal significance. Comparison of cDNA array images was performed using an average of all of the gene signals on the array (global normalization) to normalize the signal intensity between arrays. Messenger RNAs associated with GW182–mRNP complexes were considered significant if they were twofold or greater enriched over total cellular RNA. (A) Total cellular RNA; (B) mRNAs associated with GW182–mRNP complexes; (C) computer-generated overlay comparison of representative arrays A and B. Red bars represent species of mRNAs that were enriched twofold or greater as compared with total cellular RNA (genes listed in Table ​Table2).2). Green bars indicate mRNAs that were present on one or both of the arrays but were not enriched at least twofold.

Article Snippet: cDNA array analysis was performed by using Atlas Human 1.2 Arrays ( CLONTECH ) that contain 1200 cDNA segments spotted on a nylon membrane.

Techniques: Microarray, Immunoprecipitation, Software, Generated